Journal: Biomaterials

Article Title: 3D brown adipogenesis to create “Brown-Fat-in-Microstrands”

doi: 10.1016/j.biomaterials.2015.10.017

Figure Lengend Snippet: Confocal images of differentiated WT-1 cells in alginate microstrands on day 15. Terminally differentiated brown adipocytes (a–f) and undifferentiated control of brown preadipocytes in alginate microstrands without the addition of induction or differentiation media (i–o). Expression of PPARγ2 (a and j). Perilipin (b,e,k, and n). UCP1 (d and m). and (c, f, i, l, and o) DAPI for both conditions. Negative controls with secondary antibody Alexa Fluor® 488 only (g) and secondary antibody Alexa Fluor® 647 only (h). Scale bar = 100 µm.

Article Snippet: Samples were incubated with the primary antibodies of perilipin (Santa Cruz Biotechnology, Inc., Dallas, Texas), PPARγ2 (Santa Cruz Biotechnology, Inc.), and brown adipocyte-defining UCP-1 (Santa Cruz Biotechnology, Inc.), at 4°C overnight or room temperature for 1 hour.

Techniques: Control, Expressing

Journal: Biomaterials

Article Title: 3D brown adipogenesis to create “Brown-Fat-in-Microstrands”

doi: 10.1016/j.biomaterials.2015.10.017

Figure Lengend Snippet: Confocal images of ESC-derived brown adipocytes in alginate hydrogel microstrands, confirming expression of PPARγ2 (a), Perilipin (b and e), brown adipocyte-defining UCP1 (d), co-stained with DAPI (c,f, and i), and merged (d,h, and l). Negative controls were also imaged for each wavelength in order to eliminate the chance of background signal (g and h). Scale bar = 100 µm.

Article Snippet: Samples were incubated with the primary antibodies of perilipin (Santa Cruz Biotechnology, Inc., Dallas, Texas), PPARγ2 (Santa Cruz Biotechnology, Inc.), and brown adipocyte-defining UCP-1 (Santa Cruz Biotechnology, Inc.), at 4°C overnight or room temperature for 1 hour.

Techniques: Derivative Assay, Expressing, Staining

Journal: BMC Genomics

Article Title: Gene expression profile during proliferation and differentiation of rainbow trout adipocyte precursor cells

doi: 10.1186/s12864-017-3728-0

Figure Lengend Snippet: Rainbow trout primer sequences used for qPCR

Article Snippet: Twenty μg of protein were subjected to SDS-PAGE gel electrophoresis and Western blot analysis was performed using the appropriate antibodies for long-chain acyl-CoA synthetase (ACSL-1) (cat no 98925), proliferating cell nuclear antigen (PCNA) (cat no 7907), phosphoenolpyruvate carboxykinase (PEPCK) (cat no 32879), perilipin 2 (PLIN2) (cat no 32888), all from Cell Signaling Technology Inc (Beverly, MA) and PPARγ (PPARγ; cat no 7196; Santa Cruz Biotechnology).

Techniques: Sequencing

Journal: Chinese Medicine

Article Title: Zhishi Xiebai Guizhi Decoction modulates hypoxia and lipid toxicity to alleviate pulmonary vascular remodeling of pulmonary hypertension in rats

doi: 10.1186/s13020-024-01039-0

Figure Lengend Snippet: ZXGD regulated lipid metabolism to reduce cell lipid toxicity in rats with PH. A – C , H Concentration of TC, LDL-C and HDL-C (n = 6) in serum as well as decadienyl- l -carnitine (n = 3) in lung tissue were examined by ELISA and Biochemical kit. D – F Expression levels of PPARγ, PLA2 and PLIN2 in lung tissue were detected using Western blot (n = 6). G Count of lipid droplets in PASMCs was assessed with Oil red O staining, and statistical data were obtained. * p < 0.05, ** p < 0.01, *** p < 0.001 vs control, # p < 0.05, ## p < 0.01, ### p < 0.001 vs PH or PDGF-BB

Article Snippet: The methods in extraction, separation, and transferring membranes of protein samples from lung tissues and PASMCs were performed according to our previous study [ ], further incubation with the following primary antibodies: Caspase 3, Caspase 9, PLA2, PPARγ, IL-1β, IL-10 (Bioss, Beijing, China); Bax, HIF-1α, PLIN2 (Boster, Wuhan, China); PCNA (Wanleibio, Shenyang, China); IL-6 (Beyotime, Shanghai, China); Caspase 8, Bcl-2, β-actin (ABclonal, Wuhan, China) for overnight at 4 °C and subsequently incubated with secondary antibodies (goat anti-rabbit IgG, Bioss, Beijing, China) for 1h at room temperature.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Staining, Control

Journal: Chinese Medicine

Article Title: Zhishi Xiebai Guizhi Decoction modulates hypoxia and lipid toxicity to alleviate pulmonary vascular remodeling of pulmonary hypertension in rats

doi: 10.1186/s13020-024-01039-0

Figure Lengend Snippet: ZXGD modulated HIF-1α mediated pulmonary vascular remodeling. A Expression level of HIF-1α in lung tissue of rats was tested with PCR (n = 3). B Expression level of HIF-1α in PASMCs transfected with siRNA (n = 6). C Effects of ZXGD on viability of PASMCs transfected with HIF-1α siRNA was examined by MTT (n = 6). D – G Levels of IL-6, IL-10, LDL-C and decadienyl- l -carnitine in PASMCs transfected with HIF-1α siRNA. H – K Expression level of HIF-1α, Caspase-3, PCNA, PLIN2 in PASMCs transfected with HIF-1α siRNA (n ≥ 3). * p < 0.05, *** p < 0.001 vs control, # p < 0.05, ## p < 0.01, ### p < 0.001 vs PH or PDGF-BB

Article Snippet: The methods in extraction, separation, and transferring membranes of protein samples from lung tissues and PASMCs were performed according to our previous study [ ], further incubation with the following primary antibodies: Caspase 3, Caspase 9, PLA2, PPARγ, IL-1β, IL-10 (Bioss, Beijing, China); Bax, HIF-1α, PLIN2 (Boster, Wuhan, China); PCNA (Wanleibio, Shenyang, China); IL-6 (Beyotime, Shanghai, China); Caspase 8, Bcl-2, β-actin (ABclonal, Wuhan, China) for overnight at 4 °C and subsequently incubated with secondary antibodies (goat anti-rabbit IgG, Bioss, Beijing, China) for 1h at room temperature.

Techniques: Expressing, Transfection, Control

Journal: Chinese Medicine

Article Title: Zhishi Xiebai Guizhi Decoction modulates hypoxia and lipid toxicity to alleviate pulmonary vascular remodeling of pulmonary hypertension in rats

doi: 10.1186/s13020-024-01039-0

Figure Lengend Snippet: Effect of neohesperidin and naringin on cell viability, HIF-1α, Caspase3, PLIN2 in PASMCs. A – D Effects of neohesperidin and naringin on PASMCs viability were observed by MTT (n = 6). E – J Expression levels of HIF-1α, Caspase3, PLIN2 in PASMCs were evaluated by Western Blot (n ≥ 3). * p < 0.05, ** p < 0.01, *** p < 0.001 vs control, # p < 0.05, ## p < 0.01, ### p < 0.001 vs PDGF-BB. 5 represents 5 µM, 10 represents 10 µM

Article Snippet: The methods in extraction, separation, and transferring membranes of protein samples from lung tissues and PASMCs were performed according to our previous study [ ], further incubation with the following primary antibodies: Caspase 3, Caspase 9, PLA2, PPARγ, IL-1β, IL-10 (Bioss, Beijing, China); Bax, HIF-1α, PLIN2 (Boster, Wuhan, China); PCNA (Wanleibio, Shenyang, China); IL-6 (Beyotime, Shanghai, China); Caspase 8, Bcl-2, β-actin (ABclonal, Wuhan, China) for overnight at 4 °C and subsequently incubated with secondary antibodies (goat anti-rabbit IgG, Bioss, Beijing, China) for 1h at room temperature.

Techniques: Expressing, Western Blot, Control